New Pre-Clinical Study Shows Mitopure Improves Joint Health

New pre-clinical research published in the July 2022 issue of the journal Aging Cell demonstrates Mitopure’s ability to improve joint health.

Illustration of a bone joint

The science team at Timeline Nutrition’s parent company, Amazentis is at it again with another published study. Their latest pre-clinical study demonstrated their proprietary Urolithin A (UA) supplement, Mitopure's, ability to impact joint health. The article appeared in the July 2022 issue of the peer-reviewed journal Aging Cell, and it showed that Mitopure was able to improve mitochondrial health, reduce joint cartilage damage and alleviate pain in osteoarthritis.

UA and joint health

Urolithin A is a gut microbiome-derived metabolite that has been demonstrated to improve mitochondrial health by optimizing mitophagy. Mitochondrial dysfunction is commonly seen in joints with aging and with the development of osteoarthritis (OA), a fact that inspired researchers to investigate whether UA could benefit cartilage cells.

Investigators isolated cartilage cells from human knee joints of both healthy donors and those with diagnosed OA, both of which were treated with Mitopure (Urolithin A). Mitochondrial health was determined by measurements of mitochondrial respiration - a readout of mitochondrial efficiency - imaging studies and molecular biology assays.

Urolithin A > Improved Mitochondrial Health > Improved Joint Health

Urolithin A has an impact on Mitochondrial health, which in turn, improves joint health.

Primary results

The study demonstrated that Mitopure had two key effects on the cartilage of both test groups.

  • Mitopure led to significant induction of mitophagy (the recycling of damaged mitochondria).
  • Mitopure significantly increased the efficiency of mitochondria to produce energy.
Human primary
chondrocytes from: Patients with
osteoarthritis and Healthy
subjects. Treated with Urolithin A. Improved mitochondrial
 respiration and Increased Mitophagy.

In vitro

Secondary outcomes

After positive results from the first part of the trial, the second phase of the study could be initiated. This time, the researchers focused on the effect of Mitopure supplementation in an experimental model of OA. The model mimics symptoms that people with OA often experience, such as progressive cartilage damage, inflammation, and pain. Once again, significant improvements were seen.

After eight weeks of supplementation with Mitopure, the model showed:

  • Significant decreases in joint tissue degradation, as evidenced by analysis of knee joint sections.
  • A reduction in leg pain and mild anti-inflammatory response.
  • Overall better joint mitochondrial health with higher rates of mitophagy.
Experimental model 
of Osteoarthritis. Treated with Urolithin A. Increased mitophagy 
and mitochondrial content. Reduced pain, reduced inflammation and reduced cartilage degeneration.

In vivo

This study is important and exciting as it shows, for the first time, that Urolithin A can increase mitochondrial health in living joint tissue. It highlights the important role of mitophagy in maintaining healthy functioning joints throughout life.Says Davide D’Amico, first author on the paper and R&D Group Leader with Amazentis.

Why is this important?

Osteoarthritis is the most common joint disease and a leading cause of disability worldwide. There is currently no cure for this disease that causes severe pain and immobility for the people living with it. Surgery and prescription medications are currently the main therapies for managing the disease, but they do nothing to slow the progression of the disease.

This study is a big step in understanding the role of Mitopure in supporting joint health and quality of life during aging.

The results of this trial build on the growing evidence supporting the use of Mitopure for age-related conditions.  It follows on the heels of two important studies that revealed how Mitopure benefits muscle health. The Energize and Atlas trials showed that this unique compound was able to improve muscle strength and endurance in elderly and middle-aged adults. Age-related muscle loss is another contributor to disability in older adults, and Mitopure’s unique ability to target both conditions makes it an important player in aging and longevity medicine.


Jen Scheinman, MS, RDN, CDN

Written by

Senior Manager of Nutrition Affairs

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These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease. References: *Nutrition studies: 500mg Mitopure® have been shown to (1) induce gene expression related to mitochondria function and metabolism and (2) increase the strength of the hamstring leg muscle in measures of knee extension and flexion in overweight 40-65 year olds. Data from two randomized double-blind placebo-controlled human clinical trials. **Nutrition NOURISH Study: 500mg Mitopure® have been shown to deliver at least 6 times higher Urolithin A plasma levels over 24 hours (area under the curve) than 8 ounces (240ml) of pomegranate juice in a randomized human clinical trial.

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