Urolithin A is a natural compound produced when gut bacteria metabolize ellagitannins — polyphenols found in pomegranates, walnuts, and certain berries. It has emerged as one of the most studied compounds in longevity science over the last decade. What makes Urolithin A significant is its potential to trigger mitophagy — the cellular process of clearing out damaged mitochondria and replacing them with healthy ones. Mitochondrial decline is considered a key hallmark of aging.

Mitopure®, developed by Timeline after 18 years of research, is the only form of Urolithin A that has been clinically proven to activate mitophagy in humans, backed by 25 human clinical trials.

Urolithin A works at the cellular level by activating mitophagy — the body's natural process for clearing and recycling damaged mitochondria. As we age, dysfunctional mitochondria accumulate in cells, contributing to reduced energy, muscle weakness, and inflammation. By supporting mitophagy, Urolithin A helps restore a healthier population of mitochondria so cells can produce energy more effectively.

In clinical studies of Mitopure® — Timeline's clinically proven form of Urolithin A — supplementation was shown to increase mitochondrial renewal by 39% over placebo after 16 weeks, improve muscle strength by up to 12%, and enhance muscle endurance. These outcomes were observed with Mitopure® specifically, which is the only Urolithin A supplement with this level of clinical evidence.

Urolithin A is one of the most scientifically promising compounds in longevity research today. Its mechanism — activating mitophagy to clear damaged mitochondria — directly targets a key driver of cellular aging. However, not all Urolithin A supplements are equal. The clinical evidence for Urolithin A's longevity benefits comes specifically from studies on Mitopure®, Timeline's proprietary, clinically proven form.

In those trials, involving over 900 participants across 25 studies, Mitopure demonstrated meaningful improvements in mitochondrial health, muscle strength, and cellular energy. Urolithin A in its generic form has not been subjected to the same level of clinical scrutiny. For anyone looking to support long-term cellular health with confidence in the evidence, Mitopure is the only form of Urolithin A with that backing.

Urolithin A is not found directly in food. Instead, your body must produce it from ellagitannins — precursor compounds found in pomegranates and pomegranate juice, walnuts, raspberries, strawberries, and blackberries. However, eating these foods does not guarantee meaningful Urolithin A production. Only 30–40% of people have the specific gut bacteria required to convert ellagitannins into Urolithin A, and even those who can convert do so inconsistently.

Likely not since we need to rely on our gut microbiome to make it for us and only 30–40% of the population possesses the specific gut microbiome required to convert dietary precursors into Urolithin A, and even then, conversion is inconsistent. Additionally, most people are not consuming enough of the dietary precursors to make meaningful amounts of Urolithin A. Timeline demonstrated in a clinical study that you would need to drink 6 glasses of pomegranate juice per day to get the equivalent of a 500mg dose.

Because Urolithin A cannot be obtained directly from food and most people lack the gut microbiome needed to produce it reliably, supplementation with a clinically validated form is the most reliable source. Mitopure® — developed by Timeline after 18 years of research — is the only form of Urolithin A that has been clinically proven to activate mitophagy in humans. A single 500mg dose of Mitopure® delivers 6 times more Urolithin A than diet alone, bypassing the gut conversion process entirely.

Mitopure® is synthesized at over 99% purity, has received FDA GRAS (Generally Recognized as Safe) status, and is NSF Certified for Sport.

The timeline for Urolithin A's effects depends on the form taken and what outcome you're measuring. For optimal benefits, based on our clinical trials, we recommend you take Mitopure® for a minimum of 4 months to support mitochondrial function and muscular health. Many of our customers choose to stay on Mitopure indefinitely to support their long-term mitochondrial health.

These timelines are based on clinical studies of Mitopure® specifically. Generic Urolithin A supplements have not been evaluated in human trials to this standard.

Mitopure® is Timeline's proprietary Urolithin A, shown to improve mitochondrial function, which is foundational for living longer and healthier. Mitopure® is the only highly pure form of Urolithin A, clinically proven to revitalize mitochondria and change how our cells age.

Mitopure® is used in both supplements and skincare.

Yes, Mitopure® is a proprietary brand name for Urolithin A, but it differs from generic sources because it is synthesized at >99% purity, guaranteeing a precise, effective dose most cannot get from diet alone. Mitopure is clinically validated, patented, and manufactured to a higher standard than generic Urolithin A. Mitopure is also the number 1 doctor recommended Urolithin A brand.

Developed by Timeline after over 18 years of research, Mitopure® is the only clinically proven Urolithin A supplement available on the market. Mitopure® has been evaluated in multiple human trials across a diverse population, demonstrating meaningful health outcomes and verified purity, stability, and bioavailability. It has been successfully notified to the FDA as a GRAS (Generally Recognized as Safe) ingredient and is NSF Contents Certified, unlike many generic alternatives, which may lack rigorous safety testing.

Mitopure works at the foundation, improving mitochondrial quality itself rather than stimulating energy production temporarily. Unlike CoQ10, PQQ, Nicotinamide Riboside, NAD+, NMN, Mitopure primarily focuses on recycling dysfunctional mitochondria and cleaning up the old, faulty ones (mitophagy) first, preventing cellular waste accumulation. Mitopure also helps to generate new, healthier mitochondria (biogenesis).

We have performed extensive preclinical studies demonstrating the safety of Mitopure for human consumption (Heilman et al, 2017). In addition, since 2016, we have performed over 7 nutritional clinical studies, and over 12 skincare clinical studies with our Mitopure demonstrating its safety in humans (Singh et al, 2017; Liu et al, 2022; Singh et al 2022). Mitopure has also been favorably reviewed by the U.S. Food and Drug Administration (FDA) following a GRAS (generally recognized as safe) filing.

No Mitopure-related adverse events have been reported when taking Mitopure at the recommended dosage for healthy adults over 18 years of age.

If you experience any side effects from taking Mitopure, please email us at care@timelinenutrition.com or call 888-728-9846. Our team is available to help Monday through Friday, 9am to 5pm EST.

Always consult with a healthcare professional before starting supplements, especially if you have any pre-existing health conditions or are taking medications. Mitopure is not recommended for use during pregnancy, breastfeeding, or in children without medical advice.

Timeline’s Mitopure supplement is available as Softgels, , and Gummies. Explore .